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Item Open Access Contribution à l'étude phytochimique et activité biologique d'une plante médicinale Salix Purpurea(2024-06) Bahiddine, Cheima. Souadia, Zoulikha. Zabi, Karima Oum ElkheirNotre travail a été consacré à l’évaluation de certaines activités pharmacologiques (anti oxydante, insecticide et antibactérienne) et l’étude biochimique des extraits éthanolique et héxanique des feuilles de l’arbre Salix purpurea L l’ extraction par macération a montré un rendement égal à 6.42% pour l’extrait éthanolique et 5.34% pour l’extrait héxanique. Les résultats de l’analyse quantitative a permis de montrer une richesse des feuilles de cet arbre en pricipes actif, en particulier en polyphénols totaux (224.60mg/g ext sec L’évaluation de l’activité anti oxydante via le test piégeur du radical DPPH a révélé un potentiel très important pour l’extrait éthanolique avec un IC50 égale à 0.08097 tandis que l’extrait héxanique montre un m oyen potentiel (4.620). L’étude de potentiel bactérien des extraits éthanolique et héxanique contre E .coli , Staphylococus aureus, Pseudomonas aeruginosa, streptococcus thermophiles, Fusarium , Oxysporumf.sp.lycopersici et Salmonella montre que les deux extraits ont un faible activité contre les six souches bactériennes testés . Les résultats de l’activité insecticide des ‘extrai ts de S alix purpurea L montent que les extraits possèdent une activité insecticide très marquée sur la longévité des adultes de l’insecte T .confusiom Par conséquent à cet effet les‘ extraits peuvent être efficace pour la lutte contre les insectes ravageurs des denrées. Il ressort de nos résultats que les feuilles de cet arbre possèdent des propriétés thérapeutiques et insecticides très prometteuses qui capable d’être concrétisé en biopharmaceutique et pour la lutte contre les insectes ravageurs des denrées céréalières.Item Open Access Activités biologiques des extraits actifs d’une plante médicinale algérienne (Cachrys sicula L.(2024-06-12) DJOUHRI, Nadine. SOUADIA, KhawlaLes plantes médicinales sont utilisées depuis des siècles pour leurs bienfaits thérapeutiques. Cette étude examine les activités biologiques et les applications potentielles pour la santé d'une plante médicinale spécifique. Notre travail se concentre sur l'étude biologique des extraits bruts de la partie aérienne de Cachrys sicula L. de la région de M’sila. C’est une plante médicinale endémique de la région méditerranéenne plus répondu dans l’Algérie. Cachrys sicula L. est réputée pour ses propriétés biologiques prometteuses. L'objectif principal de cette étude est de mettre en lumière les activités antimicrobienne, anti-oxydante et insecticide de cette plante, tout en identifiant et quantifiant ses composés phénoliques. Les tests montrent que les extraits ont une forte activité antimicrobienne contre plusieurs souches pathogènes, une capacité anti-oxydante notable, ainsi qu'un potentiel insecticide intéressant. Le dosage des polyphénols a permis de souligner la richesse des extraits en composés phénoliques, éléments souvent corrélés avec les propriétés biologiques observées La réalisation de cette recherche a nécessité l'utilisation de l'extraction solide-liquide, la macération et la chromatographie sur couche mince (CCM). Ces méthodes ont permis une extraction efficace et une analyse précise des composés présents dans les extraits.Item Open Access Spatio-Temporal Variation Patterns of Bird Community in the Oasis Ecosystem of the North of Algerian Sahara(Université de M'sila, 2022) Lasad Chiheb; Bensaci Ettayib; Nouidjem Yassine; Hadjab RamziThe spatial and temporal variation patterns of birds were investigated in the Oasis ecosystem of the North of Algeria Sahara. This contribution aimed to investigate the poorly studied bird fauna of Bousaâda oasis. The direct observation method was used for bird counts, adopted only during the breeding period. A total of 53 species of birds from 29 families and 16 orders were assessed in the different habitats of the Oasis (palm, fruit trees and, cultivated crops). The Passeriformes order was the most abundant represented by 35 species and 16 families. The relative abundance and species richness were recorded during our study period over different seasons and thought that whole surveyed stations represent all the oasis habitats. The Boussaâda oasis holds 18 resident-breeder species and is a transit zone for many migratory birds 14 and 10 species for summer and winter migrants respectively) and11 occasional visitor ones. These results confirmed the positive effects of stations and seasons on the richness and abundance of birds of Bousaâda oasisItem Open Access Redécouverte d’Allium scaberrimum J. Serres (syn. A. pardoi Loscos) en Afrique du Nord (Algérie)(Université de M'sila, 2022) Narimène Ouafa GuechiEspèce perdue de vue depuis plus de 80 ans en Afrique du Nord, Allium scaberrimum J. Serres (Amaryllidaceae) a été observé dans plusieurs localités du nord de l’Algérie, recoupant les trois régions historiques de présence dans le pays. Malgré sa persistance à long terme, la raréfaction de son habitat exclusif justifie son statut actuel d’espèce menacée. Cela devrait encourager à une meilleure connaissance de ses populations sur le terrain ainsi qu’à des actions de conservation ex situ et de gestion puis de renforcement ou réintroduction in situItem Open Access See discussions, stats, and author profiles for this publication at: https://www.researchgate.net/publication/350567414 SURFACE WATER QUALITY ASSESSMENT IN SEMI-ARID REGION (EL HODNA WATERSHED, ALGERIA) BASED ON WATER QUALITY INDEX (WQI)(Université de M'sila, 2021) Amel FerahtiaThe present study was aimed to assess the surface water pollution sources in a semiarid region of Algeria by characterizing the physicochemical quality of the water of the main Wadis of the El Hodna watershed and determining the water quality index according to the uses of drinking water, irrigation, and aquatic life. This index is calculated in purpose to communicate information on the water quality for concerned citizens and policymakers. The calculated WQI values of the studied Wadis were higher than 100, which indicates that the waters of the El Hodna watershed are unfit for human consumption, irrigation, and aquatic life. The obtained results have shown that different types of pollution (industrial, domestic and agricultural) affect the waters of the El Hodna watershed, which lead to degrade the water quality of this aquatic ecosystem and consequently its biodiversity and public health.Item Open Access Assessment of four different methods for selecting biosurfactant producing extremely halophilic bacteria(université msila, 2021) ARIECH MouniraBiosurfactants are surface active agents with broad range commercial applications in various industries and have considerable advantages over their chemical counterparts. ln this study, drop collapse assay, oil spreading assay, emulsification assay and surface tension measurement were compared for their reliability and ease of use to screen biosurfactant producing six extremely halophilic bacteria isolated from saline soil of Chott El Hodna-M'sila (Algeria), which is considered as a thalassohaline environment. Results from screening methods revealed that, CH2 and CHS strains are potential candidates for biosurfactant production, Among these, there was a very strong negative linear correlation between the emulsification index and surface tension (r"= - 0.987) and a strong negative linear correlation between the oil spreading technique and surface tension (r8= - 0.971) and a weaker negative correlation between the drop collapse method and surface tension (r"= - 0.807), suggesting that strains highly active in any one of these methods were active in other three methods. As every method has its advantages and disadvantages, a combination of different methods is appropriate for a successful screening.Item Open Access Protective effects of Trigonella foenum-graecum crude extract over damage induced by Sreptozotocin diabetes rats(Université de M'sila, 2021) Dalila BencheikhThe seeds of Trigonella foenum-graecum (fenugreek) are used for treatment of diabetes mellitus in traditional medicine. This paper examines the protection effects of fenugreek from the damage induced by streptozotocin diabetes rats. Tannins content of T. foenum-graecum was also estimated in vitro. Normoglycemic male Wistar rats, weighing 170-250 g, were selected and randomly divided into five groups (n= 6): normal control, diabetic + TFGE (200mg/kg), diabetic+ TFGE (600mg/kg), diabetic + Glibil (3mg/kg), untreated group. Diabetes was induced after a single intraperitoneal injection of streptozotocin (50 mg/kg body weight) and Fenugreek was given every day via orogastric tube for 18 days. At the end of experiment, rats were sacarificed. Organ weight was estimated of all groups. Trigonella foenum-graecum administration significantly improved the polydipsia, polyphagia, and it also compensated weight loss of diabetic rats (P<0.05, P < 0.01). Moreover, fenugreek had a significant concentration of tannins (806.22 ±0.036 µg TAE/gE). The results revealed that fenugreek improves the damage in diabetic rats that in some ways validates the traditional use of this plant in treatment of diabetesItem Open Access AN ETHNOBOTANICAL SURVEY OF SPONTANEOUS PLANTS USED IN TRADITIONAL MEDICINE IN THE REGION OF AURES, ALGERIA(Université de M'sila, 2021) Karim BazizBackground: Like many regions in Algeria, the Aures abounds in an important ecological and floristic diversity. As a result, this region is the source of many plants used in traditional medicine. Despite this, the region is characterised by a lack of usable ethnobotanical data. Methods: In current study, semi-structured questionnaires with 701 inhabitants from September 2018 to March 2019 were conducted. Data recorded were analysed using frequency of citation (RFC) to identify the well-known and most useful species in the area. Results: This study allowed us to identify 112 medicinal spontaneous plants belonging to 47 families, of which Asteraceae, Lamiaceae, Amaranthaceae, Fabaceae, and Poaceae are the most represented and records new medicinal uses of 28 species. The leaves were the most used plant parts (28.86%), and the decoction (37.68%) represents the most frequently used mode of preparation. Also, the remedies were prepared essentially from single species (81.15%) and were used mainly in the treatment of digestive disorders (23.99%). The study has also shown that women, married people, the elderly, and illiterate people use traditional medicine much more than other categories. Conclusion: This study provides an ethnobotanical survey of medicinal plants with their frequency of citation as well as the part used, the diseases treated and the modes of application in the Aures region. It indicates that the local population has fairly extensive empirical and traditional knowledge of herbal medicine. However, this knowledge is threatened mainly because of the mode of transmission that is based on oral tradition, and urgent transcription is needed to avoid the loss of this heritage.Item Open Access Essential oil composition of aerial parts from Algerian Anacyclus monanthos subsp. cyrtolepidioides (Pomel) Humphries(Université de M'sila, 2021) Hadjer FODILThe chemical composition of the essential oil from the aerial parts of Anacyclus monanthos subsp. cyrtolepidioides (Pomel) Humphries (Asteraceae) growing in a semi-arid region of Algeria was investigated for the first time. The essential oil was obtained by hydrodistillation and fully characterized by gas chromatography-mass spectrometry (GC-MS). A total of 97 compounds were identified. The essential oil was found to be rich in trans-chrysanthenyl acetate (9.8 ± 2.0%), (E)-β-farnesene (7.4 ± 1.5%), germacrene D (6.9 ± 1.3%) and myristicin (4.8 ± 0.8%).Item Open Access EPIGENETIC EVALUATION IN ALGERIAN OPUNTIA SPECIES UNDER SALT STRESS(Université de M'sila, 2021) Hadj Kouider Boubakr; Lallouche Bahia; Ammar Boutekrabt; Ben Romdhane Mériam; Zoghlami NéjiaIn this study, we surveyed the epigenetic variations in salt stressed Opuntias belonging to four Algerian species; Opuntia species: O. ficus indica, O. amycleae, O. streptacantha, and O. engelmannii. Plants response to salt stress (0, 200 mM, 400 mM and 600 mM concentrations of Na Cl) was evaluated. Then, RAPD markers were used to apprehend the epigenetic variations at the DNA level. The analyses of the epigenetic variations were conducted using the random primers UBC228, UBC231 and UBC241 that generated 57 polymorphic markers. Results have shown that in comparison to the control plants, 13 markers were lacking in the control but induced by salt stress application. On the other hand, 02 markers were only present in the control plants. Interestingly, some markers were only induced at the highest salinity concentration (600 mM) and two bands were specific of the tolerant species O. engelmannii. In all, the 15 detected specific markers may be strongly involved in Marker-Assisted Selection studies against salt stress in cactus. Therefore, DNA sequencing of these interesting private markers may aid the identification of putative salt resistance genes, and genetic transformation procedures will subsequently facilitate their introgression in Opuntias to cope soil salinizationItem Open Access ANALYSE GÉNÉTIQUE ET VARIABILITÉ PHÉNOTYPIQUE DE LA SÉLECTION BASÉE SUR LES CARACTÈRES AGRO-MORPHO-PHYSIOLOGIQUES DE POPULATIONS AVANCÉES DE BLÉ DUR (TRITICUM DURUM DESF.), CULTIVÉES EN CONDITIONS SEMI-ARIDES(Université de M'sila, 2021) MESSAOUDI Noura; BENDERRADJI Laid; BRINI Faical; BENMAHAMMED Amar; BOUZERZOUR HamennaThis study was conducted at the Agricultural Experimental Station of the Technical Institute of Field Crops (AESTI_FC) of Sétif during the campaigns: 2016/17 and 2017/18. it concerns the behavior of eight (08) varieties of durum wheat (Triticum durum Desf.) compared to “Waha” cultivar as a control. Objective: The objective of this study is to determine the campaign, genotype, and interaction effects (genotype x campaigns) for thirteen measured variables, apart from the relative water content. Methods: The experiment was carried out in a randomized block device with 4 repetitions. The basic plot is 6 rows x 5 m long x 0.20 m inter-row spacing. The cultivation techniques recommended for the region were followed for setting up the experiment. The measurements and notations made during the culture cycle relate to the thirteen factors studied. Results : The observed variability is greater for the parameters grain yield, biomass, ear fertility, straw, and economic yields, chlorophyll content and cell integrity, for which the coefficient of variation is greater than 10%. Heritability is high for the number of kernels per ear and the chlorophyll content; the average for the weight of 1000 grains, low for grain yield, biomass, and economic yield and zero for the rest of the variables measured. The results also show that the agro-morphological characters are significantly related to each other, unlike the physiological characters which show little connection between them and with the agro-morphological characters. This suggests that among the varieties evaluated, the selection of those which are tolerant and with a high yield potential should, therefore, be done on a case-by-case basis and not on the basis of a specific physiological trait, a marker of tolerance, highly correlated with yield grain. The nine varieties evaluated are subdivided into three divergent clusters of three varieties each. Cluster C1 is made up of the least performing varieties, unlike the other two clusters which provide appreciable gains for several characteristics including grain yield, biomass, 1000 grain weight, straw yield, and ear fertility. A marked improvement in the chlorophyll content and an appreciable reduction in damage to the cell membrane by heat stress were noted. Conclusion: Following their divergence, it is suggested to use the varieties of the C2 and C3 clusters in crossing with the varieties of the C1 cluster to improve and reconcile stress tolerance and yield potential in the same genetic backgroundItem Open Access A Contribution to the Valorization of Two Medicinal Plants: Atriplex Halimus Sub. Sp. Schweinfurthii and Bunium Incrassatum, Growing in the Region of M’sila (North-East Algeria)(Université de M'sila, 2021) DEHIMI KHADIDJA; DJOUDI ZOUINA; DAHAMNA SALIHA; BOULAOUAD ANIS; MAADADI ABD RAOUF; KHENNOUF SEDDIKAtriplex halimus and Bunium incrassatum were extracted using solvents from different polarities (water, methanol, acetone and hexane). To evaluate the antioxidant abilities of the extracts, three in vitro test systems were employed: DPPH scavenging assay, β- carotene bleaching test and the reducing power assay. Extractions using acetone were the richest in polyphenols, while hexane fractions from both plants were found to contain the highest amounts of flavonoids. Tannins were more frequent in acetone fraction from AH. The best scavenging activity against DPPH radical was obtained by hexane extract from AH, followed by acetone and methanol extracts from the same plant. BI fractions showed a weak scavenging activity with IC50 values higher than 20 mg.mL-1. Acetone fractions showed the best iron reducing activity in both plants. In the BCB assay, hexane fraction from AH showed an excellent activity which was very close to that of butylated hydroxytolueneItem Open Access Chemical composition of essential oil from the aerial parts of Santolina rosmarinifolia L. a wild Algerian medicinal plant(Université de M'sila, 2021) SARRI DjamelThe analysed essential oil in this study was obtained by hydrodistillation from the aerial parts of Santolina rosmarinifolia L. (Asteraceae) collected from Hodna area of Algeria. This species is a medicinal herb traditionally used in Algeria, its essential oil has been analysed by combining GC-FID and GC-MS. The analysis led to the identification of eighty-two components, representing 91.84% of the whole composition of the sample. The main components were capillene (32.79%), 1,8-cineole (15.08%) and β-myrcene (13.98%), representing more than 61% of the total oilItem Open Access Phytochemical composition, antioxidant and wound healing activities of Teucrium polium subsp. capitatum (L.) Briq. essential oil(2020-11) Sarra Chabane; Amel Boudjelal; Edoardo Napoli; Abderrahim Benkhaled; Giuseppe RubertoTeucrium poliumis widely used in Algerian folk medicine as to treat wounds. The aim of this study was to evaluate the chemical composition, antioxidant and wound healing properties of Teucrium polium essential oil. The composition was obtained by a combination of GC-FID and GC-MS analyses. The antioxidant activity was evaluated by in vitro assays (total antioxidant capacity, DPPH and bleaching of β-carotene). The in vivo wound healing potential of an ointment containing 10% of T. polium essential oil was investigated. The main components were in this order: β-pinene, germacrene, α-pinene, myrcene, limonene, bicyclogermacrene, trans-β-guaiene, spathulenol and β-bourbonene. Teucrium polium essential oil displayed a moderate antioxidant activity. The in vivo experiments showed that 10% OEO accelerated the wound healing process in comparison with controls. This study provides a scientific rationale for the use of Teucrium polium essential oil in the treatments of wounds.Item Open Access Découverte d’Ibicella lutea (Lindl.) Van Eselt en Algérie Discovery of Ibicella lutea (Lindl.) Van Eselt in Algeria(Université de M'sila, 2020-06) Abdelouahab, BELKASSAMLes auteurs signalent la découverte à Akbou (Béjaia, NE-Algérie) et à Tissemsilt (W-Algérie) d’ibicelle jaune (Martyniaceae) originaire des zones semi‐désertiques d’Amérique du Sud.Item Open Access Potential Future Changes of the Geographic Range Size of Juniperus phoenicea in Algeria based on Present and Future Climate Change Projections(Université de M'sila, 2020-03) Abdelkrim, Arara; Yassine, Nouidjema; Rabah, Bounara; Slimane, Tabet; Yacine, KoubaMaxEnt (Maximum Entropy), a Species Distribution Modelling (SDM) method, was applied in the current study in order to assess present and future spatial distribution of the Phoenician juniper (Juniperus phoenicea). Potential future changes in the geographic range size of J. phoenicea in Algeria was determined for the two horizons 2050 and 2070 based on CCSM4 model of the IPCC. Three types of data were used in SDM namely: 21 edaphic factors, 10 topographic parameters, and 19 climatic factors. The AUC value (Area Under Curve) scored 0.966, which showed the high performance of the MaxEnt model. The most contributing variables were: total soil carbon (22.1%), Bio14: driest month precipitation (19.2%), slope (11.1%), Bio15: seasonality of precipitation (coefficient of variation) (10.3), total soil nitrogen (7%), soil available water capacity during summer (6.3%). The presence probability map obtained shows a narrowing of the favorable area of the species by about 52.5% by the year 2070. Such a result asserts the vulnerable state of this species toward the climate change, which results in altitudinal, longitudinal and latitudinal species distribution range shift as a response reflecting the becoming of unfavorable changes of the Phoenician juniper habitats. Based on these results, it is necessary to adopt necessary planning measures for the protection and conservation of the species regarding its vulnerability to climate change.Item Open Access Effects of vegetation and water seasonal variation on habitat use of herons (Aves, Ardeidae) in Tonga Lake (North-East Algeria)(Université de M'sila, 2019) Ahlam, Chettibi; Ettayib, Bensaci; Fateh, Mimeche; Soumia, DjamaiThe present study was aimed to determine the effect of some environmental factors such as water features and vegetation cover on the distribution and habitat use of herons over different seasons of the bird’s life (breeding, passage and wintering) in Tonga Lake (North East Algeria) from March 2017 to February 2018. We used remote sensing techniques to study the environmental factors variations, we adopted the normalized difference vegetation index (NDVI) to give an estimate of vegetation cover and we used the normalized difference water index (NDWI) to study the water features. In our study, we determine that the abundance and richness of herons species is largely influenced by the water features and vegetation cover. The seasonal variations in the amount of these environmental factors are known to affect the availability of various food items for herons. Eight species of herons were identified during the study period. Pearson’s Correlation Coefficient was used to examine the effect of NDVI and NDWI on the distribution of herons. The results showed that the NDVI was significantly positively correlated with richness and abundance of herons respectively (r=0.728, p<0.05) (r=0.651, p<0.05), wherease the NDWI was significantly negatively correlated with the richness and abundance of herons (r=-0.65, p<0.05) (r=-0.69, p<0.05) respectively.Item Open Access Purification andbiochemicalcharacterizationofaneworganicsolvent- tolerant chitinasefrom Paenibacillus timonensis strain LK-DZ15isolatedfrom the DjurdjuraMountainsinKabylia,Algeria(Université de M'sila, 2019) Merzouk, YahiaouiaA newextracellularchitinase(calledChiA-Pt70)wasproducedandpurifiedfromanewlyisolated Paenibacillus timonensis strain LK-DZ15.Themaximumchitinaseactivityrecordedafter44-hofincubationat30°Cwas11,500 U/mL. Pureenzymewasobtainedafterammoniumsulphateprecipitation(40–70%)followedbysequential column chromatographiesonfastperformanceliquidchromatography(FPLC)andhighperformanceliquid chromatography (HPLC).Basedonmatrixassistedlaserdesorptionionization-timeofflightmassspectrometry (MALDI-TOF/MS) analysis,thepurifiedenzymeisamonomerwithamolecularmassof70,166.11kDa.The sequence ofthe25NH2-terminal residuesofthematureChiA-70showedhighhomologywith Paenibacillus GH- 18 chitinasesfamily.OptimalactivitywasachievedatpH4.5and80°C.Thepureenzymewascompletely inhibited by p-chloromercuribenzoic acid(p-CMB), 5,5′-dithio-bis-2-nitrobenzoicacid(DTNB),and N-ethylmaleimide (NEM).Chitinaseactivitywashighoncolloidalchitin,chitinazure,glycolchitin,glycolchitosane, chitotriose, andchito-oligosaccharidewhileitdidnothydrolysechitibioseandamylose.Furthermore,thin-layer chromatography (TLC)analysisfromenzymaticcatalyzedhydrolysisofcolloidalchitinshowedthatChiA-Pt70 acted asan endo-splitting enzyme.Its Km and kcat values were0.611mgcolloidalchitin/mLand87,800s−1, respectively. Interestingly,itscatalyticefficiencywashigherthanthoseofchitinasesChiA-Mt45from Melghiribacillus thermohalophilus strain Nari2AT, ChiA-Hh59from Hydrogenophilus hirchii strain KB-DZ44, Chitodextrinase® from Streptomyces griseus, and N-acetyl-β-glucosaminidase® from Trichoderma viride. Therefore, ChiA-Pt70 exhibitedremarkablebiochemicalpropertiessuggestingthatitissuitablefortheenzymaticde- gradation ofchitin.Item Open Access Essential oils (EOs), pressurized liquid extracts (PLE) and carbon dioxide supercritical fluid extracts (SFE-CO2) from Algerian Thymus munbyanus as valuable sources of antioxidants to be used on an industrial level(Université de M'sila, 2018-03-30) Hamdi, BendifaThe aim of this study was to demonstrate the potential of extracts from Algerian Thymus munbyanus as a valuable source of antioxidants for use on an industrial level. To this end, a study was conducted on the composition and antioxidant activities of essential oils (EOs), pressurized liquid extracts (PLE) and supercritical fluid extracts (SFE-CO2) obtained from Thymus munbyanus subsp. coloratus (TMC) and subsp. munbyanus (TMM). EOs and SFECO2 extracts were analysed by GC-FID and GC×GC-TOFMS revealing significant differences. A successive extraction of the solid SFE-CO2 residue by PLE extraction with solvents of increasing polarity such as acetone, ethanol and water, was carried out. The extracts were evaluated for total phenolic content by Folin–Ciocalteu assay, while the antioxidant power was assessed by DPPH, FRAP, and ORAC assays. SFE-CO2 extracts were also analysed for their tocopherol content. The antioxidant activity of PLE extracts was found to be higher than that of SFE-CO2 extracts, and this increased with solvent polarity (water > ethanol > acetone). Overall, these results support the use of T. munbyanus as a valuable source of substances to be used on an industrial level as preservative agents.