Assessment of four different methods for selecting biosurfactant producing extremely halophilic bacteria
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Date
2021
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université msila
Abstract
Biosurfactants are surface active agents with broad range commercial applications in various industries
and have considerable advantages over their chemical counterparts. ln this study, drop collapse assay,
oil spreading assay, emulsification assay and surface tension measurement were compared for their
reliability and ease of use to screen biosurfactant producing six extremely halophilic bacteria isolated
from saline soil of Chott El Hodna-M'sila (Algeria), which is considered as a thalassohaline environment.
Results from screening methods revealed that, CH2 and CHS strains are potential candidates for
biosurfactant production, Among these, there was a very strong negative linear correlation between the
emulsification index and surface tension (r"= - 0.987) and a strong negative linear correlation between
the oil spreading technique and surface tension (r8= - 0.971) and a weaker negative correlation between
the drop collapse method and surface tension (r"= - 0.807), suggesting that strains highly active in any
one of these methods were active in other three methods. As every method has its advantages and
disadvantages, a combination of different methods is appropriate for a successful screening.
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Keywords
Biosurfactant, drop collapse, emulsification stability, extremely halophilic bacteria, oil spreading, surface tension.