Master thesis

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Browsing Master thesis by Author "Abdelatif, Maram Fethia. Fodili, Assia"

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    Phytochemical analysis and antioxidant activities of leaves extracts of two varieties of date palm (Deglet Nour and Ghars) grown in Algeria
    (UNIVERSITE MOHAMED BOUDIAF - M’SILA, 2020-09) Abdelatif, Maram Fethia. Fodili, Assia
    This study belongs to the biological studies framework, about the biological activity of phytochemical secondary metabolites, which depend on laboratory work to obtain results that reflect the extent of the existence of these biological activities and their effectiveness in plants. Our study is a ring in the chain of biological activity of date palm mainly about the antioxidant activity through the quantification of phenolic compounds in two solvent systems and measuring the value of this activity using the DPPH° scavenging assay in Deglet Nour and Ghars varieties. Through the quantification of phenolic compounds results we reached to: Firstly, the phenolic compound quantity in date palm leaves varieties is affected by the geographical area, that we have found the highest value of phenolic compounds in MeOH/H2O extract of DNM sample (DN variety). Secondly, the amount of phenolic compounds in date palm leaves varieties is affected by the variety; we have found the highest value of phenolic compounds in the GH variety of the Methanolic extract. Finely, the amount of phenolic compounds differs according to the extraction system in all varieties. Through the determination of antioxidant activity we reached: Firstly, the both varieties of date palm showed an antioxidant activity. Secondly, the value of the antioxidant activity of date palm leaves varieties is affected by the geographical area, we have reported the highest value in the Methanolic extract of DNT sample (DN variety), and this confirms the first hypothesis. Thirdly, the value of the antioxidant activity of date palm leaves varieties is affected by the variety where we have found the highest one in the GH variety of the Methanolic extract, and this confirms the second hypothesis. Then, the extraction system effects of the antioxidant activity in all studied varieties, and this confirms the third hypothesis. Finally, there are relationship and correlation between the total phenolic content and antioxidant activity in date palm leaves, and this confirms the fourth hypothesis. Through our study, we have established a set of recommendations concerning both the academic and the applied side, as follow:  Concentrate on the clarification of chemical concepts related to biological activities.  The botanical study of local date palm varieties.  Interest in study the biological activity of date palm in its various botanical parts.  Insertion of date palm leaves into alternative medicinal recipes.  Attention of updating methods for separating and purifying phenolic compounds to precisely determine the most effective compounds in the antioxidant activity. We propose for the future studies some prospects and topics which came as an extension of our study and to compensate for the deficiencies of it and repair it: Conclusion 81