EPIGENETIC EVALUATION IN ALGERIAN OPUNTIA SPECIES UNDER SALT STRESS

dc.contributor.authorHadj Kouider Boubakr
dc.contributor.authorLallouche Bahia
dc.contributor.authorAmmar Boutekrabt
dc.contributor.authorBen Romdhane Mériam
dc.contributor.authorZoghlami Néjia
dc.date.accessioned2021-07-15T09:25:33Z
dc.date.available2021-07-15T09:25:33Z
dc.date.issued2021
dc.description.abstractIn this study, we surveyed the epigenetic variations in salt stressed Opuntias belonging to four Algerian species; Opuntia species: O. ficus indica, O. amycleae, O. streptacantha, and O. engelmannii. Plants response to salt stress (0, 200 mM, 400 mM and 600 mM concentrations of Na Cl) was evaluated. Then, RAPD markers were used to apprehend the epigenetic variations at the DNA level. The analyses of the epigenetic variations were conducted using the random primers UBC228, UBC231 and UBC241 that generated 57 polymorphic markers. Results have shown that in comparison to the control plants, 13 markers were lacking in the control but induced by salt stress application. On the other hand, 02 markers were only present in the control plants. Interestingly, some markers were only induced at the highest salinity concentration (600 mM) and two bands were specific of the tolerant species O. engelmannii. In all, the 15 detected specific markers may be strongly involved in Marker-Assisted Selection studies against salt stress in cactus. Therefore, DNA sequencing of these interesting private markers may aid the identification of putative salt resistance genes, and genetic transformation procedures will subsequently facilitate their introgression in Opuntias to cope soil salinizationen_US
dc.identifier.urihttp://dspace.univ-msila.dz:8080//xmlui/handle/123456789/25009
dc.publisherUniversité de M'silaen_US
dc.titleEPIGENETIC EVALUATION IN ALGERIAN OPUNTIA SPECIES UNDER SALT STRESSen_US
dc.typeArticleen_US

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