Abstract:
The extract of Stevia leaves has been subjected to various pharmacological, clinical and toxicological investigations and results revealed interesting therapeutic applications. In addition there has been an immense interest in utilization of natural plant extracts as antimicrobial and antioxidant activity due to the increase in outbreak of food borne diseases and to minimize the health causing diseases over synthetic drugs. The purpose of this study was to collect crude extracts and investigate the presence of phytoconstituents in Stevia rebaudiana and antibacterial and antioxidant activity that serves as an affect agent to treat many diseases.
The primary phytochemical analysis of ethanolic, methanolic and water extracts revealed the presence of flavonoids, saponins, tannins, steroids, phenols compounds.
Various chemical tests with by thin-layer chromatography (TLC) showed that the major chemical constituents found in this plant were flavonoids. This result was confirmed by a quantitative analysis based on the dosage of phenolic compounds. The qualitative analysis showed that all extracts are the richest in these molecules.
In result of this study Stevia rebaudiana plant bear a potent antioxidant activity at a concentration level which is related to constituents. Their constituents scavenge free radicals and exert a protective effect against oxidative damage induced to cellular macromolecules because it has shown the presence of number of polyphenols, which are related to their chemical structure and may be responsible of the antioxidant activities
Furthermore this plant also showed promising antibacterial activity, and tests show that the plant extracts may be used effectively as an antibiotic agent against microorganisms such as Staphylococcus aureus.
Eventually, the present study reveals the presence of potential antioxidants and antimicrobial properties in the plant extract which could be exploited for pharmaceutical
